The Eukaryotic Linear Motif resource for
Functional Sites in Proteins

116 different methods used in ELM annotation

The following table holds all methods which have been used during the ELM rigourous data curation process. Furthermore, the number of ELM instances for which experimental evidence has been annotated are listed. We use methods from, and provide linkout to, the PsiMi (Proteomics Standards Initiative - Molecular Interaction) controlled vocabulary.

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IDPSIMI IDMethodBiosourceInteraction#InstancesNotes
98 MI:0004 Affinity Chromatography Technology in vivo/in vitro association 36 InteractionDetection
9 MI:0005 Alanine Scanning in vivo/in vitro/in silico 369 FeatureDetection
460 MI:0793 Amorph in vivo/in vitro/in silico association 6
37 MI:0257 Antisense RNA in vivo 3 InteractionDetection
67 MI:0007 Anti Tag Coimmunoprecipitation in vivo association 114 InteractionDetection
277 MI:0010 Beta Galactosidase Complementation 2 InteractionDetection
309 MI:0809 Bimolecular Fluorescence Complementation 8 InteractionDetection
156 MI:0969 Biolayer Interferometry 1 InteractionDetection
327 MI:0968 Biosensor 1 InteractionDetection
432 MI:0276 Blue Native Page 1 InteractionDetection
458 MI:2163 By Homology in silico association 14 ParticipantIdentification
203 MI:0225 Chromatin Immunoprecipitation Array 2 InteractionDetection
104 MI:0402 Chromatin Immunoprecipitation Assay in vivo association 2 InteractionDetection
137 MI:0091 Chromatography Technology in vitro physical association 17 InteractionDetection
18 MI:0016 Circular Dichroism in vitro association 19 InteractionDetection
65 MI:0017 Classical Fluorescence Spectroscopy in vitro association 119 InteractionDetection
405 MI:0990 Cleavage Assay 10 InteractionDetection
129 MI:0194 Cleavage Reaction in vivo/in vitro 50 InteractionDetection
23 MI:0019 Coimmunoprecipitation in vivo/in vitro association 585 InteractionDetection
16 MI:0403 Colocalization in vitro 159
146 MI:0807 Comigration In Gel Electrophoresis in vitro 7 InteractionDetection
123 MI:0404 Comigration In Non Denaturing Gel Electrophoresis in vivo association 4 InteractionDetection
132 MI:0808 Comigration In Sds Page in vitro 9 InteractionDetection
12 MI:0405 Competition Binding in vitro physical association 196 InteractionDetection
35 MI:0663 Confocal Microscopy in vivo/in vitro colocalization 71 InteractionDetection
145 MI:0027 Cosedimentation in vitro physical association 8 InteractionDetection
42 MI:0030 Cross Linking Study in vivo/in vitro direct interaction 14 InteractionDetection
134 MI:1006 Deglycosylase Assay in vivo/in vitro 33 InteractionDetection
321 MI:0033 Deletion Analysis 13 FeatureDetection
31 MI:0032 De Novo Protein Sequencing By Mass Spectrometry in vivo/in vitro association 10 ParticipantIdentification FeatureDetection
408 MI:0995 Depalmitoylase Assay 1 InteractionDetection
30 MI:0943 Detection By Mass Spectrometry in vivo/in vitro association 157 InteractionDetection
7 MI:0034 Display Technology in vivo/in vitro association 15 InteractionDetection
135 MI:0039 Edman Degradation in vitro 55 ParticipantIdentification
50 MI:0040 Electron Microscopy in vivo/in vitro association 20 InteractionDetection
345 MI:0042 Electron Paramagnetic Resonance 1 InteractionDetection FeatureDetection
436 MI:0982 Electrophoretic Mobility-based Method 1 InteractionDetection
205 MI:0413 Electrophoretic Mobility Shift Assay 11 InteractionDetection
147 MI:0412 Electrophoretic Mobility Supershift Assay association 9 InteractionDetection
131 MI:0411 Elisa in vitro direct interaction 10 InteractionDetection ParticipantIdentification
348 MI:0045 Experimental Interaction Detection 1 InteractionDetection
83 MI:0047 Far Western Blotting in vitro association 91 InteractionDetection
57 MI:1022 Field Flow Fractionation in vitro colocalization 1 InteractionDetection
342 MI:0049 Filter Binding 22 InteractionDetection
60 MI:0054 Fluorescence-activated Cell Sorting in vivo association 19 InteractionDetection
152 MI:0416 Fluorescence Microscopy colocalization 38 InteractionDetection
61 MI:0053 Fluorescence Polarization Spectroscopy in vitro association 82 InteractionDetection
143 MI:0051 Fluorescence Technology direct interaction 47 InteractionDetection
144 MI:0055 Fluorescent Resonance Energy Transfer direct interaction 12 InteractionDetection
149 MI:0728 Gal4 Vp16 Complementation in vitro physical association 7 InteractionDetection
390 MI:1003 Geranylgeranylase Assay 1 InteractionDetection
91 MI:0519 Glutathione S Tranferase Tag in vitro 624
56 MI:0367 Green Fluorescent Protein Tag in vivo 61
459 MI:0521 His Tag in vivo/in vitro/in silico association 52
216 MI:0421 Identification By Antibody 10 ParticipantIdentification
214 MI:0427 Identification By Mass Spectrometry 28 ParticipantIdentification
141 MI:0858 Immunodepleted Coimmunoprecipitation in vitro physical association 4 InteractionDetection
112 MI:0363 Inferred By Author in silico association 103 InteractionDetection ParticipantIdentification FeatureDetection
312 MI:0364 Inferred By Curator in silico association 18 InteractionDetection ParticipantIdentification FeatureDetection
148 MI:0586 Inhibitor 58
24 MI:0064 Interologs in silico association 15 InteractionDetection
202 MI:0226 Ion Exchange Chromatography 1 InteractionDetection
47 MI:0065 Isothermal Titration Calorimetry in vitro association 221 InteractionDetection
28 MI:0788 Knock Out in vivo 32
383 MI:0729 Luminescence Based Mammalian Interactome Mapping 2 InteractionDetection
86 MI:0068 Mass Detection Of Residue Modification in vitro 32 FeatureDetection
400 MI:0069 Mass Spectrometry Studies Of Complexes 3 InteractionDetection
106 MI:0944 Mass Spectrometry Study Of Hydrogen/deuterium Exchange in vivo/in vitro association 1 InteractionDetection
457 MI:1247 Microscale Thermophoresis in vitro direct interaction 4 InteractionDetection
32 MI:0071 Molecular Sieving in vivo/in vitro colocalization 20 InteractionDetection
427 MI:0072 Monoclonal Antibody Western Blot 8 ParticipantIdentification FeatureDetection
8 MI:0074 Mutation Analysis in vivo/in vitro 1535 FeatureDetection
100 MI:0573 Mutation Disrupting Interaction in vivo/in vitro 63
138 MI:1002 Myristoylase Assay in vivo/in vitro 2 InteractionDetection
150 MI:0429 Necessary Binding Site 1
246 MI:0929 Northern Blot 1 ParticipantIdentification
22 MI:0077 Nuclear Magnetic Resonance in vitro association 164 InteractionDetection FeatureDetection
142 MI:1089 Nuclear Translocation Assay in vivo 2 InteractionDetection
36 MI:0506 Over Expressed Level in vivo 10
17 Overlapping Binding Peptide in vitro 2
154 MI:0081 Peptide Array 29 InteractionDetection
63 Peptide Binding To Perpetrator in vitro 3
161 MI:0084 Phage Display 2 InteractionDetection
224 MI:1088 Phenotype-based Detection Assay 19 InteractionDetection
94 MI:0434 Phosphatase Assay in vitro enzymatic reaction 48 InteractionDetection
273 MI:1297 Phosphoamino Acid Analysis 9 FeatureDetection
53 MI:0086 Polyclonal Antibody in vitro 15 ParticipantIdentification FeatureDetection
157 MI:0435 Protease Assay 1 InteractionDetection
151 MI:0089 Protein Array direct interaction 34 InteractionDetection
26 MI:0570 Protein Cleavage in vivo/in vitro 12
73 MI:0424 Protein Kinase Assay in vitro enzymatic reaction 248 InteractionDetection
27 MI:0424 Protein Kinase Assay in vivo/in vitro enzymatic reaction 19 InteractionDetection
72 MI:0424 Protein Kinase Assay in vivo enzymatic reaction 199 InteractionDetection
69 MI:0723 Protein Microinjection in vivo 1
3 MI:0096 Pull Down in vivo/in vitro association 781 InteractionDetection
461 1260 Pyrene Label in vitro direct interaction 4 InteractionDetection
291 MI:1197 Radioimmunoassay 7 ParticipantIdentification
139 MI:0517 Radiolabel in vitro physical association 47
140 MI:0256 Rna Interference in vivo 24 InteractionDetection
136 MI:0099 Scintillation Proximity Assay in vitro physical association 2 InteractionDetection
14 MI:0101 Sequence Based Prediction in silico association 309 InteractionDetection
370 MI:1234 Silac 6 FeatureDetection
133 MI:0892 Solid Phase Assay in vitro physical association 6 InteractionDetection
454 MI:1203 Split Luciferase Complementation 1 InteractionDetection
15 MI:0105 Structure Based Prediction in silico association 41 InteractionDetection
71 MI:0372 Subcellular Preparation in vitro 14
153 MI:1008 Sumoylase Assay 3 InteractionDetection
5 MI:0107 Surface Plasmon Resonance in vitro association 115 InteractionDetection FeatureDetection
395 MI:1220 Tag Visualisation By Luciferase Assay 1 ParticipantIdentification
155 MI:0676 Tandem Affinity Purification 5 InteractionDetection
107 Two Dimensional Phosphopeptide Mapping in vitro 19
54 MI:0018 Two Hybrid in vivo association 387 InteractionDetection
93 MI:0997 Ubiquitinase Assay in vitro enzymatic reaction 41 InteractionDetection
55 MI:0113 Western Blot in vitro direct interaction 353 ParticipantIdentification FeatureDetection
4 MI:0114 X-ray Crystallography in vitro association 371 InteractionDetection FeatureDetection
357 MI:0826 X Ray Scattering 4 InteractionDetection
Please cite: ELM 2016-data update and new functionality of the eukaryotic linear motif resource. (PMID:26615199)

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