ELM - instance DEG_SCF_SKP2-CKS1_1 in sequence P46527 at position 183

Instance

Accession	Acc. Gene-, Name	Start	End	Subsequence	Logic	PDB	Organism	Length
ELMI001938	P46527 CDKN1B CDN1B_HUMAN	183	190	GSPNAGSVEQTPKKPGLRRR	TP	2AST	Homo sapiens (Human)	198

Instance evidence

Evidence class	PSI-MI	Method	BioSource	PubMed	Logic	Reliability	Notes
experimental	MI:0997	ubiquitinase assay	in vitro	Hao,2005	support	certain	InteractionDetection
experimental	MI:0074	mutation analysis	in vivo/in vitro	Hao,2005	support	certain	FeatureDetection
experimental	MI:0114	x-ray crystallography	in vitro	Hao,2005	support	certain	InteractionDetection FeatureDetection

Switches

This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:

SWTI000312:

Binding of Cyclin-dependent kinase inhibitor 1B (CDKN1B) (p27) to the SCF-Skp2 ubiquitin ligase complex requires phosphorylation of p27 (CDKN1B) at T187, and association of the F-box protein S-phase kinase-associated protein 2 (SKP2) with the regulatory Cyclin-dependent kinases regulatory subunit 1 (CKS1B). SKP2 and CKS1B together generate a composite binding site for p27 (CDKN1B). While some residues, including the phosphorylated T187, bind to CKS1B and others to SKP2, the E185 makes contact with residues of both CKS1B and SKP2.

Pathways

KEGG: The sequence P46527 is implicated in the following 19 Pathways: (color codes: This sequence=red, interacting sequence=orange)

Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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