The Eukaryotic Linear Motif resource for
Functional Sites in Proteins

o  Instance

Accession Acc. Gene-, NameStartEndSubsequenceLogic PDB OrganismLength
ELMI001665 Q8K4J6 Mkl1
MKL1_MOUSE
61 80 LKRKIRSRPERAELVRMHIL TP 2V52  
pdb:2V52 2YJE  
pdb:2YJE 2YJF  
pdb:2YJF
Mus musculus (House mouse) 964

o  Instance evidence

Evidence classPSI-MIMethodBioSourcePubMedLogicReliabilityNotes
experimental MI:0096 pull down in vivo/in vitro support certain InteractionDetection
experimental MI:0519 glutathione s tranferase tag in vitro Vartiainen,2007 support certain
experimental MI:0096 pull down in vivo/in vitro support certain InteractionDetection
experimental MI:0519 glutathione s tranferase tag in vitro Guettler,2008 support certain
experimental MI:0074 mutation analysis in vivo/in vitro Guettler,2008 support certain FeatureDetection
experimental MI:0053 fluorescence polarization spectroscopy in vitro Guettler,2008 support certain InteractionDetection
experimental MI:0367 green fluorescent protein tag in vivo Guettler,2008 support certain
experimental MI:0005 alanine scanning in vivo/in vitro/in silico Guettler,2008 support certain FeatureDetection
experimental MI:0105 structure based prediction in silico Mouilleron,2011 support certain InteractionDetection
experimental MI:0074 mutation analysis in vivo/in vitro Mouilleron,2011 support certain FeatureDetection
experimental MI:0071 molecular sieving in vivo/in vitro Mouilleron,2011 support certain InteractionDetection
experimental MI:0016 circular dichroism in vitro Mouilleron,2011 support certain InteractionDetection
experimental MI:0005 alanine scanning in vivo/in vitro/in silico Mouilleron,2011 support certain FeatureDetection
experimental MI:0403 colocalization in vitro Pawlowski,2010 support certain
experimental MI:0005 alanine scanning in vivo/in vitro/in silico Pawlowski,2010 support certain FeatureDetection
experimental MI:0074 mutation analysis in vivo/in vitro Pawlowski,2010 support certain FeatureDetection
experimental MI:0071 molecular sieving in vivo/in vitro Vartiainen,2007 support certain InteractionDetection
experimental MI:0005 alanine scanning in vivo/in vitro/in silico Vartiainen,2007 support certain FeatureDetection
experimental MI:0074 mutation analysis in vivo/in vitro Vartiainen,2007 support certain FeatureDetection
experimental MI:0074 mutation analysis in vivo/in vitro Miralles,2003 support certain FeatureDetection
experimental MI:0019 coimmunoprecipitation in vivo/in vitro Miralles,2003 support certain InteractionDetection
experimental MI:0403 colocalization in vitro Miralles,2003 support certain
experimental MI:0101 sequence based prediction in silico Miralles,2003 support certain InteractionDetection
experimental MI:0074 mutation analysis in vivo/in vitro Mouilleron,2008 support certain FeatureDetection
experimental MI:0053 fluorescence polarization spectroscopy in vitro Mouilleron,2008 support certain InteractionDetection
experimental MI:0005 alanine scanning in vivo/in vitro/in silico Mouilleron,2008 support certain FeatureDetection
experimental MI:0105 structure based prediction in silico Mouilleron,2008 support certain InteractionDetection

o  Interactions

Uniprot Id Domain family Domain Start Domain End Affinity Min/Max (µMol) Notes
(P68135) ACTS_RABIT PF00022 (Actin)
Actin
4 377 2.1/2.5 [mitab][xml]

o  Switches



This ELM instance is part of the following 1 switching mechanism annotated at the switches.ELM resource:
Please cite: The eukaryotic linear motif resource - 2018 update. (PMID:29136216)

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