The Eukaryotic Linear Motif resource for
Functional Sites in Proteins
Functional site class:
Functional site description:
Sin3 interacting domain
ELMs with same func. site: LIG_Sin3_1  LIG_Sin3_2  LIG_Sin3_3 
ELM Description:
Motif interacts with PAH2 domain in the Sin3 scaffold protein.
Pattern: [LIV]..[LM]L.AA.[FY][LI]
Pattern Probability: 1.759e-08
Present in taxon: Eukaryota
Interaction Domain:
PAH (PF02671) Paired amphipathic helix repeat (Stochiometry: 1 : 1)
PDB Structure: 1PD7
o See 4 Instances for LIG_Sin3_1
o Abstract
Gene-specific targeting of the Sin3 co-repressor complex by DNA-bound repressors is an important mechanism of gene silencing in eukaryotes. The Sin3 co-repressor is a scaffold protein able to interact with several proteins simultaneously. Sin3 specifically interacts with a diverse set of transcriptional repressors, the first of which were identified as the MAD-family members. Sin3 contains paired amphipatic helix repeat domains (PAH) that mediate protein-protein interactions. In addition, Sin3 has a histone deacetylase binding site. The SID (Sin3 interacting domain) motif specifically interacts with the PAH2 domain in Sin3. In humans, the Sin3 protein has two splice isoforms: mSin3A and mSinB. Both have the PAH2 domain and can interact with a SID-containing protein. The structure of the PAH2:MAD1 interaction has been solved (1G1E) (Brubaker,2000). The SID helix is an amphiphatic alpha helix that engages in substantial hydrophobic interactions with a deep hydrophobic groove defined by two PAH2 helices. Presumably all SID-containing proteins are targeted to a DNA region either by direct DNA binding or interaction with a DNA-binding partner, and recruit the Sin3-complex, which ultimately leads to deacetylation and repression of transcription.
o 3 selected references:

o 4 GO-Terms:

o 4 Instances for LIG_Sin3_1
(click table headers for sorting; Notes column: =Number of Switches, =Number of Interactions)
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
Q99583 MNT
3 13 MSIETLLEAARFLEWQAQQQ TP 1 Homo sapiens (Human)
P50539 MXI1
9 19 KMINVQRLLEAAEFLERRER TP 1 Homo sapiens (Human)
Q14582 MXD4
6 16 ELNSLLILLEAAEYLERRDR TP 3 Homo sapiens (Human)
Q05195 MXD1
9 19 VRMNIQMLLEAADYLERRER TP 2 Homo sapiens (Human)
Please cite: The Eukaryotic Linear Motif resource: 2022 release. (PMID:34718738)

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